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Affordable Plant Tissue Culture Part 2:

Orchids

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Carol M. Stiff, Kitchen Culture Kits, Inc., Lufkin, TX
www.kitchenculturekit.com        kck@turbonet.com


orchidseed1.jpg orchidNew38a.JPG osPhoto.BMP

Orchids are beautiful, mysterious plants which can be easily cultured in your home "lab" using materials and instructions described in my last article.  Seeds shown here were germinated under sterile conditions at my home (left photo).   The photo in the middle is from seeds I germinated about 3 years ago and are about ready to be planted in the greenhouse.  My plants are not old enough to produce a flower as shown on the right but this is an example of what you might be able to produce. 

How do you do this?  From the last article, I described simple tissue culture procedures that can be done by the hobbyist.  Using those procedures, slightly modified for the small seed of the orchid, you can become an "orchid grower". 

Note that I'm getting pretty good on growing these in sterile conditions but once they leave the "bottle", I'm in trouble.  If you are new to orchid growing, consult the experts and see how they do it.  Do not be afraid to ask for help and join a listserv if needed.  There is a new one now specific to orchid tissue culture - go to my website and click on "Listservs"  ( www.kitchenculturekit.com ) or email me at kck@turbonet.com

Here are the steps involved:


  • Get materials needed for culture: Knudson C medium or MS medium, table sugar, PPM, agar, seeds or a seed pod plus items mentioned in my last paper on home tissue culture. 
    • See this for a refresher:  http://www.kitchenculturekit.com/sivbposter.htm
    • Seeds are available, sometimes, for free for those who are rookies and are just testing the system.  Email me and I can probably find you some free non-valuable seed to play with.

  • Make your media and set up your clean box.

  • Disinfect your seeds.  There are several methods available including a "new" one I was told about on the new listserv
    • homemade filter unit
    • coffee filter method
    • sucrose and peroxide (new and easiest method!!)
    • green pod method

  • WRAP your containers to prevent contamination and at room temperature.  Some species "like" a dark incubation while others will tolerate light.
  • WAIT.  Seeds can take several weeks to several months to germinate.  Watch for contamination and discard those cultures.

  • Subculture to fresh media.  There is much information on the web about the specifics of this so do some reseach while you are waiting.  It is good to ask the source of your seeds what they recommend.

  • ENJOY.  Be prepared for the plants to take over your house.  When I started this a few years ago, the cultures took up a small space on my plant shelf in the kitchen nook.  They have since taken over my guest room for growing, not to mention other areas of the house where I prepare media, store media, process media, etc. (I'll   discuss more about this in the future). 
Knudson C media or MS media needed for orchid seed culture can be purchased from these companies which do sell to hobbyists.  I am not sure if there is a homemade media for orchid seeds but will look into it. www.phytotechlab.com
www.caissonlabs.com
www.austratec.com/au
www.kitchenculturekit.com
PPM, Plant Preservative Mixture, is not typically used in orchid seed culture but I recommend for hobby tissue culture because it helps to control contamination. www.ppm4plant-tc.com
www.phytotechlab.com
www.austratec.com/au
www.kitchenculturekit.com
(only as part of a kit; cannot sell as individual item)
Table sugar is sucrose Your kitchen
Agar health food stores

gelatin (use 1/2 tsp per 3 tablespoons liquid media; process in microwave; culture at cooler temperatures so the "jello" does not melt.

All companies mentioned above.

Seeds or a seed pod Seeds are available, sometimes, for free for those who are rookies and just testing the system.  Email me and I can probably find you some free non-valuable seed to play with

Purchase from Aaron Hicks:  ahicks51@cox.net

Knudson C Medium with Sucrose:

_____ 1 packet  Knudson C medium

_____ 1 ml PPM

_____ 5 teaspoons  sugar IF MEDIA DOES NOT INCLUDE SUCROSE

Prepare media according to instructions in manual: Add ingredients to 1 quart or 1 liter of filtered or distilled water. Mix well. Adjust pH to 5.5. Measure out 3 tablespoons to a baby food jar. Add agar to each jar before processing. Sterilize media as described in manual.

1/2 MS Medium with Sucrose:

_____ 1 packet  MS basal medium with vitamins (no sucrose)

_____ 1 ml PPM

_____ 4 tablespoons  sugar

Prepare media according to instructions in manual: Add ingredients to 2 quarts or 2 liters of filtered or distilled water. Mix well. Adjust pH to 5.5. Measure out 3 tablespoons to a baby food jar. Add agar to each jar before processing. Sterilize media as described in manual.



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© MMIV - Volume 1 Number 5 Whole Number 5  Tropical Visions September 2004

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